The Central Tuberculosis Reference Laboratory (CTRL) in Tanzania, is a laboratory that provide various services related to the diagnosis of tuberculosis (TB). The laboratory is located at the third floor of the Central Pathology Laboratory (CPL) building within Muhimbili National Hospital (MNH). The CTRL is jointly operated by the National Tuberculosis and Leprosy Control Program (NTLP) and the National Institute for Medical Research - Muhimbili Medical Research Center (NIMR - MMRC). The CTRL is accredited on Acid Fast Bacilli (AFB) smear microscopy and GeneXpert (Xpert MTB/RIF) under International Organization for Standardization (ISO) 15189 of 2012 since 2018 and has managed to maintain the accreditation for four years ever since.

The CTRL has a total of 26 employees; of whom 16 are men and 10 are women. Of the 26 employees, eight (8) are employed though NIMR and 18 through the NTLP. As per carder and qualifications; 16 are technical staff, 8 data personnel and 2 are Laboratory Attendants. Of the 16 technical staff; 1 is a holder of Doctor of Philosophy (PhD), 7 Bachelor of Science and 8 Diploma in Medical Laboratory Sciences.

The main functions of the CTRL includes: